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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts will not be yet readily available.

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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.

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This guidebook outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...

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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the absolute best final results are...

Antibodies certain to the blocking peptide not bind on the epitope to the target protein. By comparing the staining in the blocked antibody versus the antibody alone, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose users are characterised by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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